IMPORTANT DOCUMENT - PLEASE HELP!!!

[Opladybug]

"_______ ribozyme function" The word right before that looks like testing. gah but I can't really tell.

[kellylen]

The Bree Solution (that sounds REALLY important Shocked)
Objectives:
How to tansform Bree from TRAIT: POSITIVE to TRAIT:NEGATIVE

Given Facts:
1) Bree's blood contains a special Ribozyme mutated and unique only to her
2) The Genome for said ribozyme has a regular hyper mutation that changes with every generation
3) A non-functioning mutated ribozyme exists and manifests differently in every Hymn of One girl
4) This portion still functions but you cannot block it because the Complementary Strand of RNA won't combine

Theoretical Solution
1) Block the ribozyme in question by obtaining a sample of the that same ribozyme in order to___
2) Create a complementary strand of RNA
3) Q.E.D. strand will bind to ribozyme and occlude its___

Practical Solution
1) obtain blood sample of Bree
2) synthesize complimentary strand of RNA
3) inject bree with this compliementary strand
4) Q.E.D. ribozymes blocked rendered defunctual

Intended Results
1) Once synthesized and injected into bree she'll become trait negative
2) additional consequences (if any) - TBD

______ Reference Points
______ ribozyme function
a)RNAse P related -tRNA cleavage
b)_____ related - intron/exon splicing
c)______related - telomere synthesis
d)__ribozyme related - fructose 6-phosphate pathway

[TOSG]

"RNAse P Related - tRNA Cleavage"

[ohpete]

3) Q.E.D. strand will bind to ribozyme and occlude its___

Possibly: active site

active site is too long.

i would like to say energy but im not 100% sure

activity looks like it fits better. notice theres no smudge at all next to the y on the bottom, but there is on the top. and its looking more like that as i try to clean up the image.

[TOSG]

3) Q.E.D. strand will bind to ribozyme and occlude its___

Possibly: active site

active site is too long.

i would like to say energy but im not 100% sure

activity looks like it fits better. notice theres no smudge at all next to the y on the bottom, but there is on the top. and its looking more like that as i try to clean up the image.

Looks like "efficacy" to me, but I'm not sure.

[anniid]

try ironing the paper. on a really really low setting...
and don't let sarah do it, haha.
that should help an itty bitty bit.

[kellylen]

active site is too long.

i would like to say energy but im not 100% sure

activity looks like it fits better. notice theres no smudge at all next to the y on the bottom, but there is on the top. and its looking more like that as i try to clean up the image.

Looks like "efficacy" to me, but I'm not sure.

that was my 2nd thought

[ohpete]

3) Q.E.D. strand will bind to ribozyme and occlude its___
our posibilities seem to be: activity, energy, and efficiency.
the problem is, they all fit.

[Aponi]

given Facts:
1) Bree's blood contains a special Ribozyme mutated and unique only to her
2) Genome for said ribozyme has a regular hyper mutation that changes with every generation
3) A non-function(al?) (mutated?) ribozyme exists and manifests differently in every Hymn of One girl
4) This portion still functions but you cannot blend because the complimentary Strand of RNA won't combine

Theoretical Solution
1) Block the ribozyme in question by obtaining a sample of the that same ribozyme in order to___
2) Create a supplementary strand of RNA
3) Q.E.D. strand will bind to ribozyme and occlude its (-y)

Practical Solution
1) obtain blood sample of Bree
2) synthesize complimentary strand of RNA
3) inject bree with this compliementary strand
4) Q.E.D. ribozymes blocked rendered defunctive

Intended Results
1) Once synthesized and injected into bree she'll become trait negative
2) additional consequences (if any) - TBD

(P or B-?) (-i-ty?) Reference Points
_______Understanding ribozyme function
_______-tRNA cleavage
_______ related - intron/exon splicing
__________ - telomere synthesis
________ - fructose 6-phosphate pathway


Is what I have so far.

[ohpete]

agh. cmon guys. we can get this.

[kellylen]

yes yes yes mutated makes sense

[Opladybug]

3) Q.E.D. strand will bind to ribozyme and occlude its___
our posibilities seem to be: activity, energy, and efficiency.
the problem is, they all fit.

I'd like to put in a vote for activity.

[ohpete]

given Facts:
1) Bree's blood contains a special Ribozyme mutated and unique only to her
2) Genome for said ribozyme has a regular hyper mutation that changes with every generation
3) A non-function(al?) (mutated?) ribozyme exists and manifests differently in every Hymn of One girl
4) This portion still functions but you cannot blend because the complimentary Strand of RNA won't combine

Theoretical Solution
1) Block the ribozyme in question by obtaining a sample of the that same ribozyme in order to___
2) Create a supplementary strand of RNA
3) Q.E.D. strand will bind to ribozyme and occlude its (-y)

Practical Solution
1) obtain blood sample of Bree
2) synthesize complimentary strand of RNA
3) inject bree with this compliementary strand
4) Q.E.D. ribozymes blocked rendered defunctive

Intended Results
1) Once synthesized and injected into bree she'll become trait negative
2) additional consequences (if any) - TBD

(P or B-?) (-i-ty?) Reference Points
_______Understanding ribozyme function
_______-tRNA cleavage
_______ related - intron/exon splicing
__________ - telomere synthesis
________ - fructose 6-phosphate pathway


Is what I have so far.

Probability Reference Points? I'm just throwing anything out there at this point.

[Aponi]

3) Q.E.D. strand will bind to ribozyme and occlude its___
our posibilities seem to be: activity, energy, and efficiency.
the problem is, they all fit.

I'm sure Spencer will be able to figure it out. He's the RNA specialist XD

[kellylen]

well occlude means to shut off if that helps