Spencer the solution!

[kellylen]

Hey hun

Here is the tentative solution to the papers you gave us today

The Bree Solution (that sounds REALLY important Shocked)
Objectives:
How to tansform Bree from TRAIT: POSITIVE to TRAIT:NEGATIVE

Given Facts:
1) Bree's blood contains a special Ribozyme mutated and unique only to her
2) The Gene for said ribozyme has a regulatory hyper mutation that changes with every generation
3) A non-functioning mutated ribozyme exists and manifests differently in every Hymn of One girl
4) This portion still functions but you cannot block it because the Complementary Strand of RNA won't copy

Theoretical Solution
1) Block the ribozyme in question by obtaining a sample of the that same ribozyme in order to copy it
2) Create a complementary strand of RNA
3) Q.E.D. strand will bind to ribozyme and occlude its copy

Practical Solution
1) obtain blood sample of Bree
2) synthesize complimentary strand of RNA
3) inject bree with this compliementary strand
4) Q.E.D. ribozymes blocked rendered ineffectual

Intended Results
1) Once synthesized and injected into bree she'll become trait negative
2) additional consequences (if any) - TBD

Bree Trait Reference Points
Theories concerning ribozyme function
a)RNAse P related -tRNA cleavage
b)splicosome related - intron/exon splicing
c)Hammerhead related - telomere synthesis
d)GlmS ribozyme related - fructose 6-phosphate pathway

this is what we think so far. and most of it is 99% sure on

[Samara]

To be honst, I think this solution was posted in haste. For example:

defunctual

That's not actually a word...

I believe that the word is "ineffectual"

[kellylen]

fixed it. the point is that the foundation is there. the important parts are there. a word here or there will not... or well should not throw off the whole thing

[anniid]

See!?
You should have posted that paper sooner... within 2-3 hours we had a solution. (It's correctness is um... relative...)
Alright. Check our solution and give us another puzzle!!!
Come on... you know you want to, Spencer.

[ohpete]

theres my copy. im too exauhsted to check it against yours for the moment kelly. ive got to get some food in me. but here it is as a back up.

The Bree Solution (that sounds REALLY important )
Objectives:
How to transform Bree from TRAIT: POSITIVE to TRAIT: NEGATIVE

Given Facts:
1) Bree's blood contains a special ribozyme mutated and unique only to her.
2) The gene for said ribozyme has a regulatory hyper mutation that changes with every generation.
3) A non-functioning mutated (?) ribozyme exists and manifests differently in every Hymn of One girl.
4) This portion still functions but you cannot block it because the complimentary strands of RNA won't copy.

Theoretical Solution
1) Block the ribozyme in question by obtaining a sample of that same ribozyme in order to copy it.
2) Create complimentary strand of RNA.
3) Q.E.D. strand will bind to ribozyme and occlude its copy.

Practical Solution
1) Obtain blood sample of Bree.
2) Synthesize complimentary strand of RNA
3) Inject Bree with this complementary strand.
4) Q.E.D. ribozymes blocked will be rendered ineffectual.

Intended Results
1) Once synthesized and injected into Bree she'll become trait negative
2) Additional consequences (if any) - TBD

Bree Trait Reference Points
The steps concerning ribozyme function
RNAse P related -tRNA cleavage
Splicosome related - intron/exon splicing
Hammerhead or Telomerase(?) related - telomere synthesis
GlmS ribozyme related - fructose 6-phosphate pathway

[SivartAlappes]

See!?
You should have posted that paper sooner... within 2-3 hours we had a solution. (It's correctness is um... relative...)
Alright. Check our solution and give us another puzzle!!!
Come on... you know you want to, Spencer.

1 hour and 41 minutes, actually.

[kellylen]

See!?
You should have posted that paper sooner... within 2-3 hours we had a solution. (It's correctness is um... relative...)
Alright. Check our solution and give us another puzzle!!!
Come on... you know you want to, Spencer.

1 hour and 41 minutes, actually.

actually i think its a lot less than that

[emerrs11]

I agree Kelly! I am 99.99% sure. I trust these answers as much as I trust Neutragena!

[SivartAlappes]

See!?
You should have posted that paper sooner... within 2-3 hours we had a solution. (It's correctness is um... relative...)
Alright. Check our solution and give us another puzzle!!!
Come on... you know you want to, Spencer.

1 hour and 41 minutes, actually.

actually i think its a lot less than that

Well, the 1 hour and 41 minutes is the time between when Spencer posted the letter, and when you posted the solution.

[Aponi]

See!?
You should have posted that paper sooner... within 2-3 hours we had a solution. (It's correctness is um... relative...)
Alright. Check our solution and give us another puzzle!!!
Come on... you know you want to, Spencer.

1 hour and 41 minutes, actually.

actually i think its a lot less than that

It was posted around 9:30, and by the time I was done with it enough to look at the clock it was around 11. Sounds about right to me

And I have to say, WTG US! Puzzle-solving ftw
Seriously, props to everyone who helped (Yes Kelly, even Travis XD)

[QtheC]

3) Q.E.D. strand will bind to ribozyme and occlude its copy

4) Q.E.D. ribozymes blocked rendered ineffectual

I can neither confirm nor deny ever having E.D.'d, but I'm willing to try it if it will help the cause, and someone can provide me with step by step instructions and the necessary ribozymes. As long as it is no harder than Sea-Monkeys, I should have or be able to improvise any laboratory equipment needed (tupperware, microwave, blender, power tools, hybrid bicycle, fire, nails, ziplock bags, Dr. Pepper, batteries). Let me know if I have left anything important off the list - I might have it.

[anniid]

See!?
You should have posted that paper sooner... within 2-3 hours we had a solution. (It's correctness is um... relative...)
Alright. Check our solution and give us another puzzle!!!
Come on... you know you want to, Spencer.

1 hour and 41 minutes, actually.

actually i think its a lot less than that

well his first post was at 7:30--something.
then we finished at 9:17

[thoughtssopoetic]

1 hour and 41 minutes, actually.

actually i think its a lot less than that

well his first post was at 7:30--something.
then we finished at 9:17

I am now cursing the fact that I have night class from 6-9:30
gah.

[BlackCatonMars]

I am now cursing the fact that I have night class from 6-9:30
gah.

I was eating dinner and then watching the movie Sneakers and didn't get back online until I was of no use...and I've been waiting for weeks for a chance to help out! Oh well.

[marlasinger]

wooooooord! Kelly we posted that theory that i was talking about last night here http://www.lonelygirl15.com/forum/viewtopic.php?t=12203. Take a look and tell me what you think - things to remember - no double stranded rna (rNAi), just complementary and active strands. Someone's already mentioned that it might be science fiction.