IMPORTANT DOCUMENT - PLEASE HELP!!!

[SivartAlappes]

4) This portion still functions but you cannot block it because the Complementary Strand of RNA won't combine

i think its blend not block it.

I think you're right. given what the strand DOES, "Blend" makes MUCH more sense than "Block"

[kellylen]

______related - telomere synthesis

Replication of linear chromosomes presents a special problem.

DNA polymerase can only synthesize a new strand of DNA as it moves along the template strand in the 3' –> 5' direction. This works fine for the 3' –> 5' strand of a chromosome as the DNA polymerase can move uninterruptedly from an origin of replication until it meets another bubble of replication or the end of the chromosome. However, synthesis using the 5' –> 3' strand as the template has to be discontinuous. When the replication fork opens sufficiently, DNA polymerase can begin to synthesize a section of complementary strand — called an Okazaki fragment — working in the opposite direction. Later, DNA ligase stitches the Okazaki fragments together.

^^taken from a site about telomore

lol funny random story i was in biochemistry last semsester and my asian friend asked me if i wanted to see his Okazaki ;D haha

and that thing about telomeres i would say is more about introns and exons.

anddd blend doesnt make much sense. you cant blend RNA together. you need enzymes to cut and piece together it

[Aponi]

So.

Bree has a special mutated ribozyme that is different from everyone else's. Every HoO girl? Do they get something at birth and only they have it, and not people like us? Bree's mutated ribozyme is 'special' to the HoO, it's the one they want.

The rest of it:
3) A non-functioning mutated(?) ribozyme exists and manifests differently in every Hymn of One girl.
4) This portion still functions but you cannot block it because the complimentary strands of RNA won't combine

Theoretical Solution
1) Block the ribozyme in question by obtaining a sample of the that same ribozyme in order to ____
2) Create complimentary strand of RNA.
3) Q.E.D. strand will bind to ribozyme and occlude its activity/energy/effeciency(?).

Practical Solution
1) Obtain blood sample of Bree .
2) Synthesize complimentary strand of RNA
3) Inject Bree with this compliementary strand.
4) Q.E.D. ribozymes blocked will be rendered defunctual.

Intended Results
1) Once synthesized and injected into Bree she'll become trait negative
2) Additional consequences (if any) - TBD

Makes sense by itself.
Except TBD? I wonder what that means?

[kellylen]

tbd = to be determined

The Bree Solution (that sounds REALLY important Shocked)
Objectives:
How to tansform Bree from TRAIT: POSITIVE to TRAIT:NEGATIVE

Given Facts:
1) Bree's blood contains a special Ribozyme mutated and unique only to her
2) The Gene for said ribozyme has a regular hyper mutation that changes with every generation
3) A non-functioning mutated ribozyme exists and manifests differently in every Hymn of One girl
4) This portion still functions but you cannot block it because the Complementary Strand of RNA won't combine

Theoretical Solution
1) Block the ribozyme in question by obtaining a sample of the that same ribozyme in order to copy it
2) Create a complementary strand of RNA
3) Q.E.D. strand will bind to ribozyme and occlude its activity

Practical Solution
1) obtain blood sample of Bree
2) synthesize complimentary strand of RNA
3) inject bree with this compliementary strand
4) Q.E.D. ribozymes blocked rendered defunctual

Intended Results
1) Once synthesized and injected into bree she'll become trait negative
2) additional consequences (if any) - TBD

______ Reference Points
Theories concerning ribozyme function
a)RNAse P related -tRNA cleavage
b)splicosome related - intron/exon splicing
c)Hammerhead related - telomere synthesis
d)GlmS ribozyme related - fructose 6-phosphate pathway

[FeministPsych]

Aponi - TBD usually means "To Be Determined"

Meaning they don't know what side effects this "treatment" could have on Bree besides making her Trait Negative.

Meaning it could be dangerous for her. Or it could be totally fine.

[Samara]

defunctual

I'm pretty sure thats actually "ineffectual"

[ohpete]

The Bree Solution (that sounds REALLY important )
Objectives:
How to transform Bree from TRAIT: POSITIVE to TRAIT:NEGATIVE

Given Facts:
1) Bree's blood contains a special ribozyme mutated and unique only to her.
2) The gene for said ribozyme has a regular hyper mutation that changes with every generation.
3) A non-functioning mutated(?) ribozyme exists and manifests differently in every Hymn of One girl.
4) This portion still functions but you cannot block it because the complimentary strands of RNA won't combine.

Theoretical Solution
1) Block the ribozyme in question by obtaining a sample of the that same ribozyme in order to ____
2) Create complimentary strand of RNA.
3) Q.E.D. strand will bind to ribozyme and occlude its activity/energy/effeciency(?).

Practical Solution
1) Obtain blood sample of Bree .
2) Synthesize complimentary strand of RNA
3) Inject Bree with this compliementary strand.
4) Q.E.D. ribozymes blocked will be rendered defunctual.

Intended Results
1) Once synthesized and injected into Bree she'll become trait negative
2) Additional consequences (if any) - TBD

Biological Trait(?) Reference Points
The steps concerning ribozyme function
RNAse P related -tRNA cleavage
Splicosome related - intron/exon splicing
______related - telomere synthesis
GlmS ribozyme related - fructose 6-phosphate pathway

[FeministPsych]

Darn it, thought I actually contributed something useful here, but got beaten to the punch...

(Goes back to lurking)

[kellylen]

guys we rock we are alomst done!

just 1 more thing and a few thigns to work out the broken parts we arent too sur eof

[Aponi]

4) This portion still functions but you cannot block it because the Complementary Strand of RNA won't combine

i think its blend not block it.

I think you're right. given what the strand DOES, "Blend" makes MUCH more sense than "Block"

In context, it seems blend is more likely. Saying you can't block it because it won't combine? If it won't combine it's blocking itself.

Yeah trying to be an English major in a science thing like this is useless sometimes XD

Oh, and thanks on TBD. I'm useless with acronyms. Not joke. That's why I want to kill TCC right now.

[marlasinger]

[kellylen]

so maybe the complementary strand wont copy

things having to do with DNA and RNA and genes do NOT blend

[ohpete]

tbd = to be determined

The Bree Solution (that sounds REALLY important Shocked)
Objectives:
How to tansform Bree from TRAIT: POSITIVE to TRAIT:NEGATIVE

Given Facts:
1) Bree's blood contains a special Ribozyme mutated and unique only to her
2) The Gene for said ribozyme has a regular hyper mutation that changes with every generation
3) A non-functioning mutated ribozyme exists and manifests differently in every Hymn of One girl
4) This portion still functions but you cannot block it because the Complementary Strand of RNA won't combine

Theoretical Solution
1) Block the ribozyme in question by obtaining a sample of the that same ribozyme in order to copy it
2) Create a complementary strand of RNA
3) Q.E.D. strand will bind to ribozyme and occlude its activity

Practical Solution
1) obtain blood sample of Bree
2) synthesize complimentary strand of RNA
3) inject bree with this compliementary strand
4) Q.E.D. ribozymes blocked rendered defunctual

Intended Results
1) Once synthesized and injected into bree she'll become trait negative
2) additional consequences (if any) - TBD

______ Reference Points
Theories concerning ribozyme function
a)RNAse P related -tRNA cleavage
b)splicosome related - intron/exon splicing
c)Hammerhead related - telomere synthesis
d)GlmS ribozyme related - fructose 6-phosphate pathway

are we pretty sure its activity now?
im having my gf clean it up with photoshop and update me since mine is broken. im doing what i can guys.

[kellylen]

i think it may be copy now XD it makes sense if you change combine to copy

[Aponi]

so maybe the complementary strand wont copy

things having to do with DNA and RNA and genes do NOT blend

definitly begins with a C and it looks short. If you say copy I say I copy.
God i'm retarded. (And for a second I was like 'do not compute? XD)

it's saying it WONT blend anyway. So doesn't that agree with you