IMPORTANT DOCUMENT - PLEASE HELP!!!

[Aponi]

Ew. I just killed a skeeter on my comp screen. Nasty.

And I WANT to print this out but i'm out of ink, maybe someone should print Marla's reference point version, it might be easier to read that way.

[krmurr87]

Maybe it's somebodies name? Or references from a book?

[marlasinger]

from some random sites:

" One way to repair mRNA is to use ribozymes, RNA molecules that act as 'molecular scissors' by catalysing the cleavage of mRNA at sequence-specific sites."

"Targeting a mutation discovered in an affected dog, they engineered a ribozyme that splices off the mutation-containing 3? section of the mRNA and replaces it with the wild-type sequence. This technique is called ribozyme-mediated trans-splicing."

http://www.vetscite.org/publish/items/001060/index.html


knowing the function and theories of ribozymes will make filling in those blanks a LOT easier, guys.

[tazman2087]

Im pretty sure its "Bree Trait Reference Points"

[ohpete]

Looks like we're this far:

The Bree Solution (that sounds REALLY important )
Objectives:
How to transform Bree from TRAIT: POSITIVE to TRAIT:NEGATIVE

Given Facts:
1) Bree's blood contains a special ribozyme mutated and unique only to her.
2) Genome for said ribozyme has a regular hyper mutation that changes with every generation.
3) A non-functioning mutated(?) ribozyme exists and manifests differently in every Hymn of One girl.
4) This portion still functions but you cannot block it because the complimentary strands of RNA won't combine.

Theoretical Solution
1) Block the ribozyme in question by obtaining a sample of the that same ribozyme in order to ____
2) Create complimentary strand of RNA.
3) Q.E.D. strand will bind to ribozyme and occlude its activity/energy/effeciency(?).

Practical Solution
1) Obtain blood sample of Bree .
2) Synthesize complimentary strand of RNA
3) Inject Bree with this compliementary strand.
4) Q.E.D. ribozymes blocked will be rendered defunctual.

Intended Results
1) Once synthesized and injected into Bree she'll become trait negative
2) Additional consequences (if any) - TBD

______ Reference Points
The steps concerning ribozyme function
RNAse P related -tRNA cleavage
Splicosome related - intron/exon splicing
______related - telomere synthesis
GlmS ribozyme related - fructose 6-phosphate pathway

[kellylen]

from some random sites:

" One way to repair mRNA is to use ribozymes, RNA molecules that act as 'molecular scissors' by catalysing the cleavage of mRNA at sequence-specific sites."

"Targeting a mutation discovered in an affected dog, they engineered a ribozyme that splices off the mutation-containing 3? section of the mRNA and replaces it with the wild-type sequence. This technique is called ribozyme-mediated trans-splicing."

http://www.vetscite.org/publish/items/001060/index.html


knowing the function and theories of ribozymes will make filling in those blanks a LOT easier, guys.

which is why i got most of it the first time through

we still need to knowww why it needs to be made complementary in order to: osadoiadoaihBLANK

[marlasinger]

I guess what I'm trying to say is..

Instead of focusing on the words we DON'T have, let's work with the words we DO have. Words like...Intron. Exon. fructose-6-phosphate. Things we can research. If we can't get the sentence, we can get the relationship between ribozymes and these substances. okay?

[SivartAlappes]

can it occlude its copy? or does that make no sense?

In chemistry, occlude means to incorporate something by absorbtion... so yes, in this case (we're making a synthetic copy of the ribozyme) the original not only CAN occlude it's copy, it must.

[Aponi]

Pag? Sociat? Reference Points.

Just saying what the words look like. I think the last letter of the first one is a g.

And if I need to know about RNA i'm kinda screwed.

[kellylen]

well whats his face has gotten most of the bottom things and they are right. i did some research and each time they posted something i was about to say that XD

[Raven_Night]

______related - telomere synthesis

Replication of linear chromosomes presents a special problem.

DNA polymerase can only synthesize a new strand of DNA as it moves along the template strand in the 3' –> 5' direction. This works fine for the 3' –> 5' strand of a chromosome as the DNA polymerase can move uninterruptedly from an origin of replication until it meets another bubble of replication or the end of the chromosome. However, synthesis using the 5' –> 3' strand as the template has to be discontinuous. When the replication fork opens sufficiently, DNA polymerase can begin to synthesize a section of complementary strand — called an Okazaki fragment — working in the opposite direction. Later, DNA ligase stitches the Okazaki fragments together.

^^taken from a site about telomore

[kellylen]

can it occlude its copy? or does that make no sense?

In chemistry, occlude means to incorporate something by absorbtion... so yes, in this case (we're making a synthetic copy of the ribozyme) the original not only CAN occlude it's copy, it must.

then occlude the copy makes sense in order to block the other one to stop the whole thing ... thing

[simplykimmie13]

4) This portion still functions but you cannot block it because the Complementary Strand of RNA won't combine

i think its blend not block it.

[TOSG]

I THINK that c) should be "Hammerhead related - telomere synthesis", but the lettering is damn near impossible to make out.

Also, it's "the gene," not "genome," for given fact 2).

[ohpete]

*slams keyboard*
photoshop still wont open, and now im sitting here trying searches for anything i can think of, because google hasnt helped me to fill in the blanks any more at this point. [though i know a lot more about this stuff now.]